Analysis of low molecular weight GTPase activity in endothelial cell cultures

Methods Enzymol. 2008;443:285-98. doi: 10.1016/S0076-6879(08)02014-4.

Abstract

GTPases control a myriad of cellular processes, including cell migration, proliferation, polarity, and cell adhesion, which includes cell-cell junction regulation. Angiogenesis requires many of these diverse cellular events to occur appropriately. In this chapter, we describe the techniques for in vitro assessment of GTPase activity in endothelial cells grown in monolayer culture as a model system for studying their role during angiogenesis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Blotting, Western
  • Cells, Cultured
  • Electrophoresis, Polyacrylamide Gel
  • Endothelial Cells / cytology
  • Endothelial Cells / metabolism*
  • Endothelial Cells / physiology
  • GTP Phosphohydrolases / chemistry
  • GTP Phosphohydrolases / metabolism*
  • Humans
  • Molecular Weight
  • Neovascularization, Physiologic / physiology
  • cdc42 GTP-Binding Protein / chemistry
  • cdc42 GTP-Binding Protein / metabolism
  • rac GTP-Binding Proteins / chemistry
  • rac GTP-Binding Proteins / metabolism
  • rap1 GTP-Binding Proteins / chemistry
  • rap1 GTP-Binding Proteins / metabolism

Substances

  • GTP Phosphohydrolases
  • cdc42 GTP-Binding Protein
  • rac GTP-Binding Proteins
  • rap1 GTP-Binding Proteins