The effect of blastomere biopsy on preimplantation mouse embryo development and global gene expression

Francesca E Duncan; Paula Stein; Carmen J Williams; Richard M Schultz

doi:10.1016/j.fertnstert.2008.07.1710

The effect of blastomere biopsy on preimplantation mouse embryo development and global gene expression

Fertil Steril. 2009 Apr;91(4 Suppl):1462-5. doi: 10.1016/j.fertnstert.2008.07.1710. Epub 2008 Sep 6.

Authors

Francesca E Duncan¹, Paula Stein, Carmen J Williams, Richard M Schultz

Affiliation

¹ Center for Research on Reproduction and Women's Health, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

Abstract

The blastomere biopsy procedure does not affect preimplantation embryo development or global patterns of gene expression in a mouse model of Preimplantation Genetic Testing (PGT). However, zona breaching, which is inherent to the blastomere biopsy procedure, causes significant premature and sometimes abnormal hatching.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Biopsy
Blastomeres / pathology*
Embryo Transfer / methods*
Embryonic Development / physiology*
Female
Gene Expression Regulation, Developmental / physiology*
Mice
Models, Animal
Preimplantation Diagnosis / methods
Sperm Injections, Intracytoplasmic
Time Factors

Abstract

Publication types

MeSH terms

Grants and funding