Determination of cardiac troponin I in the blood and heart of calves with foot-and-mouth disease

J Vet Diagn Invest. 2008 Sep;20(5):598-605. doi: 10.1177/104063870802000510.


The current study was designed to determine the changes of the cardiac troponin I (cTnI) expression in blood and tissue during the myocardial degeneration in calves with foot-and-mouth disease (FMD). Seventeen crossbred calves presenting pathological signs for FMD confirmed by viral analysis were studied. A biochemistry panel and immunohistochemistry were performed on 17 diseased calves and 7 calves used as controls. Creatine kinase (CK), CK-myocardial band (CK-MB), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities were analyzed for both groups. Cardiac troponin I levels were measured by a commercially available enzyme-linked immunosorbent assay kit. Mean cTnI (14.8 +/- 1.9 ng/ml) concentration and CK (573 +/- 407 U/l), CK-MB (238 +/- 37 U/l), AST (84 +/- 7), and LDH (298 +/- 29 U/l) activities were higher in FMD cases compared with controls. Immunohistochemistry revealed loss or depletion of cTnI expression in myocardium of all cases. None of the 7 controls showed loss of cTnI expression. Increased serum cTnI concentration correlated with myocardial injury and loss of cTnI immunolabeling in cardiomyocytes of calves with FMD.

MeSH terms

  • Animals
  • Cattle
  • Cattle Diseases / blood
  • Cattle Diseases / epidemiology
  • Cattle Diseases / pathology*
  • Disease Outbreaks
  • Foot-and-Mouth Disease / epidemiology
  • Foot-and-Mouth Disease / metabolism*
  • Foot-and-Mouth Disease / pathology
  • Immunohistochemistry
  • Myocarditis / pathology
  • Myocarditis / veterinary*
  • Myocardium / pathology*
  • Troponin I / blood*
  • Troponin I / metabolism*
  • Turkey / epidemiology


  • Troponin I