Protein-facilitated ribozyme folding and catalysis

Nucleic Acids Symp Ser (Oxf). 2008:(52):67-8. doi: 10.1093/nass/nrn034.


In vivo, large RNAs rely on proteins to fold to their native conformation. In the case of the S. cerevisiae group II intron ai5 gamma, the DEAD-box protein Mss116 has been shown to promote the formation of the catalytically active structure. However, it is a matter of debate whether it does this by stabilizing on-pathway intermediates or by disrupting misfolded structures. Here we present the available experimental evidence to distinguish between those mechanisms and discuss the possible interpretations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Catalysis
  • DEAD-box RNA Helicases / genetics
  • DEAD-box RNA Helicases / metabolism*
  • Introns*
  • Molecular Sequence Data
  • Mutation
  • Nucleic Acid Conformation
  • RNA Splicing*
  • RNA, Catalytic / chemistry*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*


  • RNA, Catalytic
  • Saccharomyces cerevisiae Proteins
  • MSS116 protein, S cerevisiae
  • DEAD-box RNA Helicases