Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, (52), 477-8

New Strategies for DNA Polymerase Library Screening

Affiliations

New Strategies for DNA Polymerase Library Screening

Ramon Kranaster et al. Nucleic Acids Symp Ser (Oxf).

Abstract

Engineered enzymes are of increasing importance for a plethora of biotechnical applications. Especially DNA polymerases are workhorses in biochemical technologies in particular the polymerase chain reaction (PCR), cDNA cloning procedures, genome sequencing and in diagnostic applications. DNA polymerase mutant libraries can be used for the screening of non-standard reaction conditions or substrates e.g. the efficient amplification of difficult templates like ancient DNA. We are convinced that these fascinating enzymes can be optimized and custom-made for a specific application to result in more robust and reliable systems. To our knowledge, all known screening methods for DNA polymerase mutants are focused and thus limited to the screening of a single reaction or one new function. We developed improved strategies for multiplexed DNA polymerase screening that will be presented.

Similar articles

See all similar articles

Cited by 1 PubMed Central articles

MeSH terms

Feedback