Immunomodulatory effect of Antrodia camphorata mycelia and culture filtrate

J Ethnopharmacol. 2008 Nov 20;120(2):196-203. doi: 10.1016/j.jep.2008.08.011. Epub 2008 Aug 19.


Aim of the study: Antrodia camphorata, a precious folkloric medicinal mushroom, has been used to treat tumorigenic diseases in Taiwan. This study was to investigate the innate immunity augmentation effects of different fractions prepared from hot water extracts of submerged cultured Antrodia camphorata (AC).

Materials and methods: The cytokine induction potency of AC fraction in diluted peripheral blood culture was measured by ELISA. The effects of AC fraction on phagocytic activity and CD11b expression were measured by the ingestion of FITC-labeled Escherichia coli and by labeling with PE-labeled CD11b monoclonal antibody, respectively, using flow cytometry. The molecular mass of hot water-soluble polysaccharides and content of adenosine in AC fraction were determined by gel permeation chromatography (GPC) and HPLC, respectively.

Results: The mycelia fraction, Fr. M II, and culture filtrate fractions, Fr. E II and Fr. E III, showed the strongest TNF-alpha and IL-6 induction effect as a function of their concentration. These fractions (20mug/ml) also showed marked activity in enhancing phagocytosis in human polymorphonuclear neutrophils (PMN) and monocytes. In parallel, the expression of CD11b, an early marker of PMN activation, was also up-regulated dose-dependently. Composition analysis suggested that immunomodulatory effect of mycelia is mainly attributed to the 10-20kDa polysaccharides and adenosine.

Conclusions: These results provide evidences that Antrodia camphorata can modulate innate immunity and may serve as an adjuvant for tumor treatment.

MeSH terms

  • Adenosine / administration & dosage
  • Adenosine / immunology
  • Adenosine / isolation & purification
  • Adult
  • Antrodia / chemistry*
  • CD11b Antigen / drug effects*
  • CD11b Antigen / immunology
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Culture Media
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Hot Temperature
  • Humans
  • Immunologic Factors / administration & dosage
  • Immunologic Factors / immunology*
  • Immunologic Factors / isolation & purification
  • Interleukin-6 / metabolism
  • Medicine, Chinese Traditional
  • Mycelium / chemistry
  • Neutrophils / immunology
  • Phagocytosis / drug effects
  • Polysaccharides / administration & dosage
  • Polysaccharides / immunology
  • Polysaccharides / isolation & purification
  • Taiwan
  • Tumor Necrosis Factor-alpha / drug effects
  • Tumor Necrosis Factor-alpha / metabolism
  • Up-Regulation / drug effects


  • CD11b Antigen
  • Culture Media
  • Immunologic Factors
  • Interleukin-6
  • Polysaccharides
  • Tumor Necrosis Factor-alpha
  • Adenosine