Reshaping of the endoplasmic reticulum limits the rate for nuclear envelope formation

J Cell Biol. 2008 Sep 8;182(5):911-24. doi: 10.1083/jcb.200805140.

Abstract

During mitosis in metazoans, segregated chromosomes become enclosed by the nuclear envelope (NE), a double membrane that is continuous with the endoplasmic reticulum (ER). Recent in vitro data suggest that NE formation occurs by chromatin-mediated reorganization of the tubular ER; however, the basic principles of such a membrane-reshaping process remain uncharacterized. Here, we present a quantitative analysis of nuclear membrane assembly in mammalian cells using time-lapse microscopy. From the initial recruitment of ER tubules to chromatin, the formation of a membrane-enclosed, transport-competent nucleus occurs within approximately 12 min. Overexpression of the ER tubule-forming proteins reticulon 3, reticulon 4, and DP1 inhibits NE formation and nuclear expansion, whereas their knockdown accelerates nuclear assembly. This suggests that the transition from membrane tubules to sheets is rate-limiting for nuclear assembly. Our results provide evidence that ER-shaping proteins are directly involved in the reconstruction of the nuclear compartment and that morphological restructuring of the ER is the principal mechanism of NE formation in vivo.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Cell Line, Tumor
  • Chromatin / metabolism
  • Chromatin / ultrastructure
  • Endoplasmic Reticulum / metabolism*
  • Endoplasmic Reticulum / ultrastructure
  • Green Fluorescent Proteins / analysis
  • Humans
  • Kinetics
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Myelin Proteins / genetics
  • Myelin Proteins / metabolism
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Nogo Proteins
  • Nuclear Envelope / metabolism*
  • Nuclear Envelope / ultrastructure
  • Recombinant Fusion Proteins / analysis
  • Transcription Factor DP1 / genetics
  • Transcription Factor DP1 / metabolism

Substances

  • Carrier Proteins
  • Chromatin
  • Membrane Proteins
  • Myelin Proteins
  • Nerve Tissue Proteins
  • Nogo Proteins
  • RTN3 protein, human
  • RTN4 protein, human
  • Recombinant Fusion Proteins
  • Transcription Factor DP1
  • Green Fluorescent Proteins