MicroRNA-27a functions as an oncogene in gastric adenocarcinoma by targeting prohibitin

Cancer Lett. 2009 Jan 18;273(2):233-42. doi: 10.1016/j.canlet.2008.08.003. Epub 2008 Sep 13.

Abstract

MicroRNAs (miRNAs) may function as oncogenes or tumor suppressors. Here, we show that miR-27a is up-regulated in human gastric adenocarcinoma. Suppression of miR-27a inhibits gastric cancer cell growth. Subsequently, prohibitin is identified as a potential miR-27a target, combining bioinformatics and microarray analysis. EGFP report experiment also confirms that the 3' untranslated region (3'UTR) of prohibitin carries the directly binding site of miR-27a. After knockdown of miR-27a in gastric cancer cells, mRNA level and protein level of prohibitin are both elevated. Down-regulation of prohibitin by miR-27a may explain why suppression of miR-27a can inhibit gastric cancer cell growth, further supporting that miR-27a functions as an oncogene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Adenocarcinoma / metabolism*
  • Binding Sites
  • Cell Line, Tumor
  • Cell Transformation, Neoplastic
  • Disease Progression
  • Gene Expression Regulation, Neoplastic*
  • Green Fluorescent Proteins / metabolism
  • Humans
  • MicroRNAs / genetics*
  • MicroRNAs / physiology*
  • Oncogenes
  • Repressor Proteins / chemistry*
  • Stomach Neoplasms / metabolism*

Substances

  • 3' Untranslated Regions
  • MIRN27 microRNA, human
  • MicroRNAs
  • Repressor Proteins
  • prohibitin
  • Green Fluorescent Proteins