Mitochondrial depolarisation and oxidative stress in rheumatoid arthritis patients

Clin Biochem. 2008 Nov;41(16-17):1396-401. doi: 10.1016/j.clinbiochem.2008.08.072. Epub 2008 Aug 29.


Objectives: To investigate mitochondrial membrane integrity, lipid peroxidation and cytotoxicity in peripheral lymphocytes (PL) from rheumatoid arthritis (RA) patients.

Design and methods: South African black RA patients (HIV(-)) were recruited into the study. Mitochondrial membrane potential (Deltapsi(m)) was analysed in PL using the JC-1 dye distribution assay and flow cytometry. Correlations between Deltapsi(m) and clinical parameters were tested for statistical significance. Cytotoxicity (LDH) and lipid peroxidation (thiobarbituric acid reactive substances (TBARS)) was also determined.

Results: Our findings show significantly elevated levels of cytotoxicity (p=0.0029) and lipid peroxidation (p=0.0030) in RA. A significantly higher percentage of circulating PL contained depolarised mitochondria (p=0.0003) which correlated with disease activity and C-reactive protein levels in patients. Collapse of Deltapsi(m) also negatively correlated to absolute lymphocyte counts (r=-0.4041; p=0.0197).

Conclusion: These findings suggest a possible role for mitochondrial membrane alterations in the pathology of RA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis, Rheumatoid / blood
  • Arthritis, Rheumatoid / physiopathology*
  • Benzimidazoles
  • Carbocyanines
  • Humans
  • L-Lactate Dehydrogenase / blood
  • Lymphocyte Count
  • Membrane Potential, Mitochondrial / physiology*
  • Oxidative Stress*


  • Benzimidazoles
  • Carbocyanines
  • 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine
  • L-Lactate Dehydrogenase