Response regulator YycF essential for bacterial growth: X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif

Toshihide Okajima; Akihiro Doi; Ario Okada; Yasuhiro Gotoh; Katsuyuki Tanizawa; Ryutaro Utsumi

doi:10.1016/j.febslet.2008.09.007

Response regulator YycF essential for bacterial growth: X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif

FEBS Lett. 2008 Oct 15;582(23-24):3434-8. doi: 10.1016/j.febslet.2008.09.007. Epub 2008 Sep 26.

Authors

Toshihide Okajima¹, Akihiro Doi, Ario Okada, Yasuhiro Gotoh, Katsuyuki Tanizawa, Ryutaro Utsumi

Affiliation

¹ Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan. tokajima@sanken.osaka-u.ac.jp

PMID: 18789936
DOI: 10.1016/j.febslet.2008.09.007

Abstract

A response regulator YycF and its cognate sensor kinase YycG constitute the two-component signal transduction system essential for growth of Gram-positive bacteria with a low GC content. We have determined the X-ray crystal structure of the effector domain of Bacillus subtilis YycF involved in DNA binding. The structure, containing a winged helix-turn-helix motif, was found to be very similar to that of the response regulator PhoB from Escherichia coli. Specific binding of YycF to the PhoB-regulated alkaline phosphatase promoter was also demonstrated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / genetics
Bacillus subtilis / growth & development*
Bacillus subtilis / metabolism
Bacterial Proteins / chemistry*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Base Sequence
Crystallography, X-Ray
DNA / chemistry*
DNA / metabolism
Gene Expression Regulation, Bacterial*
Helix-Turn-Helix Motifs* / genetics
Molecular Sequence Data
Promoter Regions, Genetic
Protein Structure, Tertiary

Substances

Bacterial Proteins
YycF protein, Bacteria
PhoB protein, Bacteria
DNA