Metagenomic screening for bleomycin resistance genes

Appl Environ Microbiol. 2008 Nov;74(21):6803-5. doi: 10.1128/AEM.00873-08. Epub 2008 Sep 12.

Abstract

A metagenomic library of activated sludge was screened for bleomycin resistance genes. Two genes were identified that differed greatly from each other, from the genes of bleomycin-producing actinomycetes, and from those of clinical isolates. Therefore, the nonclinical environment is a rich reservoir of new resistance elements, and metagenomics can be used to sample the resistome rapidly.

MeSH terms

  • Antibiotics, Antineoplastic / pharmacology*
  • Bacteria / genetics*
  • Bleomycin / pharmacology*
  • Drug Resistance, Bacterial
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Homology
  • Sewage / microbiology*

Substances

  • Antibiotics, Antineoplastic
  • Sewage
  • Bleomycin

Associated data

  • GENBANK/AB300377
  • GENBANK/AB331169