Reconstitution of iron oxidase from sulfur-grown Acidithiobacillus ferrooxidans

Appl Environ Microbiol. 2008 Nov;74(21):6808-10. doi: 10.1128/AEM.00787-08. Epub 2008 Sep 12.

Abstract

The iron oxidation system from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270 cells was reconstituted in vitro. Purified rusticyanin, cytochrome c, and aa(3)-type cytochrome oxidase were essential for reconstitution. The iron-oxidizing activity of the reconstituted system was 3.3-fold higher than that of the cell extract from which these components were purified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acidithiobacillus / enzymology*
  • Azurin / isolation & purification
  • Azurin / metabolism
  • Cytochromes c / isolation & purification
  • Cytochromes c / metabolism
  • Electron Transport Complex IV / isolation & purification
  • Electron Transport Complex IV / metabolism
  • Iron / metabolism*
  • Oxidoreductases / isolation & purification*
  • Oxidoreductases / metabolism*

Substances

  • rusticyanin
  • Azurin
  • Cytochromes c
  • Iron
  • Oxidoreductases
  • Electron Transport Complex IV