A simple polymerase chain reaction-based method for the construction of recombinase-mediated cassette exchange donor vectors

Genetics. 2008 Nov;180(3):1763-6. doi: 10.1534/genetics.108.094508. Epub 2008 Sep 14.

Abstract

Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the PhiC31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Drosophila Proteins / genetics*
  • Drosophila melanogaster / genetics*
  • Female
  • Gene Targeting
  • Genetic Vectors*
  • Integrases / metabolism
  • Male
  • Polymerase Chain Reaction / methods*
  • Recombinases / physiology*
  • Transgenes / physiology*

Substances

  • Drosophila Proteins
  • Recombinases
  • Integrases