Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor beta in HEK293 cells

Biochem Biophys Res Commun. 2008 Nov 21;376(3):478-82. doi: 10.1016/j.bbrc.2008.09.017. Epub 2008 Sep 13.


Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor beta, (PPARbeta). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARbeta in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARbeta. Furthermore, PPARbeta also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Gene Library
  • Humans
  • Immunoprecipitation
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Molecular Chaperones
  • Muscle Proteins / genetics
  • Muscle Proteins / metabolism*
  • PPAR-beta / antagonists & inhibitors*
  • PPAR-beta / genetics
  • PPAR-beta / metabolism
  • Protease Inhibitors / pharmacology
  • Transcription, Genetic*
  • Two-Hybrid System Techniques


  • Membrane Proteins
  • Molecular Chaperones
  • Muscle Proteins
  • PPAR-beta
  • PSMG1 protein, human
  • Protease Inhibitors