Function and regulation of claudins in the thick ascending limb of Henle

Pflugers Arch. 2009 May;458(1):77-88. doi: 10.1007/s00424-008-0589-z. Epub 2008 Sep 16.


The thick ascending limb (TAL) of Henle mediates transcellular reabsorption of NaCl while generating a lumen-positive voltage that drives passive paracellular reabsorption of divalent cations. Disturbance of paracellular reabsorption leads to Ca(2+) and Mg(2+) wasting in patients with the rare inherited disorder of familial hypercalciuric hypomagnesemia with nephrocalcinosis (FHHNC). Recent work has shown that the claudin family of tight junction proteins form paracellular pores and determine the ion selectivity of paracellular permeability. Importantly, FHHNC has been found to be caused by mutations in two of these genes, claudin-16 and claudin-19, and mice with knockdown of claudin-16 reproduce many of the features of FHHNC. Here, we review the physiology of TAL ion transport, present the current view of the role and mechanism of claudins in determining paracellular permeability, and discuss the possible pathogenic mechanisms responsible for FHHNC.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Bartter Syndrome / physiopathology
  • Biological Transport, Active
  • Calcium / metabolism
  • Claudins
  • Gitelman Syndrome / genetics
  • Gitelman Syndrome / physiopathology
  • Humans
  • Loop of Henle / physiology*
  • Magnesium / metabolism
  • Membrane Proteins / genetics*
  • Membrane Proteins / physiology*
  • Nephrocalcinosis / genetics*
  • Nephrocalcinosis / physiopathology
  • Receptors, Calcium-Sensing / physiology
  • Tight Junctions / physiology


  • CASR protein, human
  • Claudins
  • Cldn19 protein, mouse
  • Membrane Proteins
  • Receptors, Calcium-Sensing
  • claudin 16
  • Magnesium
  • Calcium