The dynamics of the expression of C/EBP mRNA in the adult rat liver lobulus qualifies it as a pericentral mRNA

FEBS Lett. 1991 Aug 19;288(1-2):133-7. doi: 10.1016/0014-5793(91)81019-5.


A hybridocytochemical approach has been applied to establish whether the gene for the C/EBP mRNA might be involved in the topographical regulation of gene expression in adult rat liver. To that end the spatial distribution of the mRNA of C/EBP has been compared to that of the mRNAs of glutamine synthetase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glucokinase (GK) in normal adult livers, in livers from dexamethasone-treated animals and in livers from starved animals refed with glucose for 4 h. In normal rat liver, in situ hybridization with a probe for C/EBP mRNA revealed a low density of apparently homogeneously distributed grains, indicating low levels of C/EBP mRNA. In contrast, the livers of the experimentally-treated animals revealed a zonal distribution of the mRNA of C/EBP with the highest density of grains around the central venules. The dynamics of the pattern of expression of C/EBP mRNA are virtually identical to that of the GK mRNA. These data qualify C/EBP mRNA as a pericentral mRNA and suggest a role for the C/EBP protein in the topographical regulation of the expression of the GK mRNA.

MeSH terms

  • Animals
  • Base Sequence
  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins / genetics*
  • Dexamethasone / pharmacology
  • Gene Expression Regulation / physiology*
  • Glucokinase / genetics
  • Glutamate Synthase / genetics
  • Liver / drug effects
  • Liver / metabolism*
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Nucleic Acid Hybridization
  • Phosphoenolpyruvate Carboxykinase (GTP) / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Starvation
  • Transcription Factors / genetics*
  • Transcription, Genetic


  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • Transcription Factors
  • Dexamethasone
  • Glutamate Synthase
  • Glucokinase
  • Phosphoenolpyruvate Carboxykinase (GTP)