Taurine modulates calcium influx under normal and ototoxic conditions in isolated cochlear spiral ganglion neurons

Pharmacol Rep. Jul-Aug 2008;60(4):508-13.

Abstract

The effect of taurine on calcium homeostasis of isolated cochlear spiral ganglion neurons under normal and ototoxic conditions was investigated using fluo-3 calcium imaging. Sole application of taurine (15 mM) induced an increase in intracelluar Ca(2+) concentration ([Ca(2+)](i)), which was largely inhibited either by the application of an L-type calcium-channel blocker nifedipine or a calcium-free medium. Preincubation with 1 mM gentamicin induced an inhibition of the high K(+)-evoked elevation of [Ca(2+)](i). Short-term exposure to taurine prevented this inhibition. The results suggested that taurine at this concentration was able to increase [Ca(2+)](i) mainly by calcium influx through L-type calcium channels in isolated spiral ganglion neurons and to antagonize gentamicin-induced inhibition of calcium elevation evoked by high K(+) by its calcium homeostatic effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Guinea Pigs
  • Hair Cells, Auditory, Outer / drug effects*
  • Hair Cells, Auditory, Outer / metabolism
  • Homeostasis / drug effects
  • Spiral Ganglion / drug effects*
  • Spiral Ganglion / metabolism
  • Taurine / pharmacology*

Substances

  • Taurine
  • Calcium