Stimulation of dorsal root ganglion neurons activity by pancreatic cancer cell lines

Cell Biol Int. 2008 Dec;32(12):1530-5. doi: 10.1016/j.cellbi.2008.08.022. Epub 2008 Aug 30.

Abstract

Stimulation of mice dorsal root ganglion neurons (DRGNs) activity by human pancreatic cancer (PanCa) cell line Mia PaCa-2 and its potential molecule mechanism has been investaged. DRGNs were cultured alone or along with the MIA PaCa-2. The effects of MIA PaCa-2 to DRGNs were determined by neurofilament (NF) immunocytochemical and Nissl staining. ELISA was used to detect the concentration of insulin-like growth factor-1 (IGF-1) in the culture supernatant. Cyton size, neurite outgrowth and neuronal activity in the experimental group were greater than in the control groups. However, the concentration of IGF-1 in the supernatants was not significantly different from those in the blank and non-cultured medium groups. In the presence of MIA PaCa-2 cell line, cyton size, neurite outgrowth and neuronal activity were enhanced, which may provide more routes for the invasion of cancer cells along nerves.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Carcinoma / metabolism
  • Carcinoma / physiopathology
  • Cell Communication / physiology*
  • Cell Enlargement / drug effects
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Cell Survival / physiology
  • Culture Media, Conditioned / pharmacology
  • Ganglia, Spinal / cytology
  • Ganglia, Spinal / drug effects
  • Ganglia, Spinal / metabolism*
  • Growth Cones / drug effects
  • Growth Cones / metabolism*
  • Growth Cones / ultrastructure
  • Humans
  • Insulin-Like Growth Factor I / metabolism*
  • Mice
  • Neoplasm Invasiveness / physiopathology*
  • Pancreatic Neoplasms / metabolism
  • Pancreatic Neoplasms / physiopathology
  • Peripheral Nerves / metabolism
  • Peripheral Nerves / pathology
  • Peripheral Nerves / physiopathology
  • Sensory Receptor Cells / cytology
  • Sensory Receptor Cells / drug effects
  • Sensory Receptor Cells / metabolism*

Substances

  • Culture Media, Conditioned
  • Insulin-Like Growth Factor I