Gene expression changes induced by space flight in single-cells of the fern Ceratopteris richardii

Planta. 2008 Dec;229(1):151-9. doi: 10.1007/s00425-008-0817-y. Epub 2008 Sep 20.


This work describes a rare high-throughput evaluation of gene expression changes induced by space flight in a single plant cell. The cell evaluated is the spore of the fern Ceratopteris richardii, which exhibits both perception and response to gravity. cDNA microarray and Q RT-PCR analysis of spores germinating in microgravity onboard NASA space shuttle flight STS-93 revealed changes in the mRNA expression of roughly 5% of genes analyzed. These gene expression changes were compared with gene expression changes that occur during gravity perception and response in animal cells and multicellular plants. Our data contribute to a better understanding of the impact of space flight conditions, including microgravity, on cellular growth and development, and provide insights into the adaptive strategies of individual cells in response to these conditions.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Arabidopsis / genetics
  • Down-Regulation / genetics
  • Gene Expression Regulation, Plant*
  • Genes, Plant
  • Oligonucleotide Array Sequence Analysis
  • Peroxidase / genetics
  • Peroxidase / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plant Roots / genetics
  • Pteridaceae / cytology*
  • Pteridaceae / enzymology
  • Pteridaceae / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Space Flight*
  • Weightlessness


  • Plant Proteins
  • Peroxidase