In vitro and in vivo validation of ligA and tarI as essential targets in Staphylococcus aureus

Karin Streker; Tina Schäfer; Christoph Freiberg; Heike Brötz-Oesterhelt; Jörg Hacker; Harald Labischinski; Knut Ohlsen

doi:10.1128/AAC.00548-07

In vitro and in vivo validation of ligA and tarI as essential targets in Staphylococcus aureus

Antimicrob Agents Chemother. 2008 Dec;52(12):4470-4. doi: 10.1128/AAC.00548-07. Epub 2008 Sep 22.

Authors

Karin Streker¹, Tina Schäfer, Christoph Freiberg, Heike Brötz-Oesterhelt, Jörg Hacker, Harald Labischinski, Knut Ohlsen

Affiliation

¹ Universität Würzburg, Institut für Molekulare Infektionsbiologie, Röntgenring 11, 97070 Würzburg, Germany.

Abstract

A conditional expression system has been developed using the isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible Pspac promoter to validate essential genes of Staphylococcus aureus in vivo. The system has been applied to prove the essentiality of ligA and to evaluate the function of tarI, which was found to be essential in vitro but not in vivo.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Abscess / microbiology
Animals
Bacterial Proteins / genetics*
Culture Media
DNA Ligases / genetics*
Disease Models, Animal
Female
Gene Expression Regulation, Bacterial
Genes, Essential*
Humans
Isopropyl Thiogalactoside / pharmacology
Mice
Mutation
Staphylococcal Skin Infections / microbiology*
Staphylococcus aureus / drug effects
Staphylococcus aureus / genetics*
Staphylococcus aureus / growth & development*

Substances

Bacterial Proteins
Culture Media
Isopropyl Thiogalactoside
DNA Ligases