Control of the blue fluorescent protein with advanced evolutionary pulse shaping

Biochem Biophys Res Commun. 2008 Nov 28;376(4):733-7. doi: 10.1016/j.bbrc.2008.09.075. Epub 2008 Sep 24.


We demonstrate optical coherent control of the two-photon fluorescence of the blue fluorescent protein (BFP), which is of interest in investigations of protein-protein interactions. In addition to biological relevance, BFP represents an interesting target for coherent control from a chemical perspective due to its many components of highly nonexponential fluorescence decay and low quantum yield resulting from excited state isomerization. Using a genetic algorithm with a multiplicative (rather than ratiometric) fitness parameter, we are able to control the ratio of BFP fluorescence to second-harmonic generation without a considerable drop in the maximized signal. The importance of linear chirp and power-scaling on the discrimination process is investigated in detail.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Fluorescence
  • Fluorescence Resonance Energy Transfer
  • Luminescent Proteins / chemistry*
  • Luminescent Proteins / genetics
  • Photons


  • Luminescent Proteins