Liquid chromatographic analysis of guanidino compounds using furoin as a new fluorogenic reagent

J Pharm Biomed Anal. 2008 Nov 4;48(3):754-9. doi: 10.1016/j.jpba.2008.07.017. Epub 2008 Jul 30.


Furoin, a benzoin analogue, was examined as novel fluorogenic reagent for the selective and sensitive LC determination of various guanidines after pre-column derivatization. The derivatization reaction was carried out at 100 degrees C for 5 min to give adducts that were separated on a Phenomenex Synergi MAX-RP column and detected at lambda(em)=410 nm with lambda(ex)=325 nm. The reagent showed to be useful both for determining together arginine (Arg) and creatine (CT) in dietary supplements under elution isocratic conditions and for the simultaneous analysis of a variety of guanidines in biological samples (human plasma and urine) under elution gradient conditions. The detection limits ranged from 7 to 25 fmol. Recovery studies showed good results for all determined guanidino compounds (85.6-106.2%; R.S.D.=1.1-6.2%).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzoin / analogs & derivatives*
  • Benzoin / chemistry*
  • Calibration
  • Chromatography, Liquid / methods*
  • Dietary Supplements / analysis
  • Furans
  • Guanidines / analysis*
  • Guanidines / blood
  • Guanidines / urine
  • Humans
  • Indicators and Reagents / chemistry*
  • Molecular Structure
  • Reference Standards
  • Sensitivity and Specificity
  • Time Factors


  • 3,3'-furoin
  • Furans
  • Guanidines
  • Indicators and Reagents
  • Benzoin