Abstract
A development of a rapid and sensitive LC-MS/MS method for the simultaneous detection of active ingredients of the euthanasic veterinarian drug Tanax mixture is described. The method proposed, with a retention time of few minutes (6 min) was developed for an equine serum sample with solid-phase extraction (S.P.E). This S.P.E. procedure has been revealed useful for the determination of very low concentrations of Tanax analytes (0.05-1 ng/ml). The method was validated in terms of specificity/selectivity, sensitivity, recovery and precision.
MeSH terms
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Amides / analysis*
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Amides / chemistry
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Amides / toxicity*
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Animals
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Chromatography, Liquid / veterinary*
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Cyclohexanes / analysis
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Cyclohexanes / toxicity
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Drug Combinations
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Drug Stability
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Euthanasia*
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Flow Injection Analysis / methods
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Horses
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Molecular Structure
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Quaternary Ammonium Compounds / analysis*
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Quaternary Ammonium Compounds / chemistry
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Quaternary Ammonium Compounds / toxicity*
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Reproducibility of Results
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Sensitivity and Specificity
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Solid Phase Extraction / methods
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Spectrometry, Mass, Electrospray Ionization / methods
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Tandem Mass Spectrometry / veterinary*
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Tetracaine / analysis*
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Tetracaine / chemistry
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Tetracaine / toxicity*
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Time Factors
Substances
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Amides
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Cyclohexanes
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Drug Combinations
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Quaternary Ammonium Compounds
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mebenzonium iodide
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Tetracaine
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embutramide
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T 61