Rabbit neuromedin U-25: lack of conservation of a posttranslational processing site

Regul Pept. 1991 Apr 25;33(2):191-8. doi: 10.1016/0167-0115(91)90213-z.

Abstract

The rabbit small intestine contains neuromedin U-like immunoreactivity (22 pmol/g wet tissue weight) that was resolved into a single major molecular form by reversed-phase HPLC. The primary structure of the peptide was established as: Phe-Pro-Val-Asp-Glu-Glu-Phe-Gln-Ser-Pro10-Phe-Gly-Ser-Arg-Ser-Arg- Gly-Tyr-Phe- Leu20-Phe-Arg-Pro-Arg-Asn.NH2. In rabbit neuromedin U, the Arg16-Arg17 dibasic residue processing site that is found in pig and dog neuromedin U-25 is replaced by Arg-Gly, but this potential monobasic processing site is not utilized by cleavage enzyme(s) in the intestine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromatography, High Pressure Liquid
  • Digestive System / chemistry
  • Molecular Sequence Data
  • Neuropeptides / isolation & purification*
  • Neuropeptides / metabolism
  • Protein Processing, Post-Translational
  • Rabbits

Substances

  • Neuropeptides
  • neuromedin U 25