Quantification of sunitinib in human plasma by high-performance liquid chromatography-tandem mass spectrometry

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Oct 15;874(1-2):84-8. doi: 10.1016/j.jchromb.2008.09.007. Epub 2008 Sep 12.

Abstract

A rapid, sensitive and specific method was developed and validated using LC/MS/MS for determination of sunitinib in human plasma. Sample preparation involved a liquid-liquid extraction by the addition of 0.2mL of plasma with 4.0mL tert-butyl-methyl-ether extraction solution containing 25ng/mL of the internal standard clozapine. Separation of compounds was achieved on a C18 (50mmx2.1mm i.d., 3.5microm) analytical column using a mobile phase consisting of acetonitrile/H20 (65:35, v/v) containing 0.1% formic acid and isocratic flow at 0.150mL/min for 3min. The analytes were monitored by tandem-mass spectrometry with electrospray positive ionization. Linear calibration curves in human plasma were generated over the range of 0.2-500ng/mL with values for the coefficient of determination of >0.9950. Within- and between day precision and accuracy were < or =10%. The method was applied to the quantitation of sunitinib in plasma samples from a patient receiving daily oral therapy with sunitinib.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Humans
  • Indoles / blood*
  • Indoles / chemistry
  • Molecular Structure
  • Pyrroles / blood*
  • Pyrroles / chemistry
  • Reproducibility of Results
  • Sunitinib
  • Tandem Mass Spectrometry / methods*

Substances

  • Indoles
  • Pyrroles
  • Sunitinib