Use of ProteinChip technology for identifying biomarkers of parasitic diseases: the example of porcine cysticercosis (Taenia solium)

Exp Parasitol. 2008 Dec;120(4):320-9. doi: 10.1016/j.exppara.2008.08.013. Epub 2008 Sep 11.


Taenia solium cysticercosis is a significant public health problem in endemic countries. The current serodiagnostic techniques are not able to differentiate between infections with viable cysts and infections with degenerated cysts. The objectives of this study were to identify specific novel biomarkers of these different disease stages in the serum of experimentally infected pigs using ProteinChip technology (Bio-Rad) and to validate these biomarkers by analyzing serum samples from naturally infected pigs. In the experimental sample set 30 discriminating biomarkers (p<0.05) were found, 13 specific for the viable phenotype, 9 specific for the degenerated phenotype and 8 specific for the infected phenotype (either viable or degenerated cysts). Only 3 of these biomarkers were also significant in the field samples; however, the peak profiles were not consistent among the two sample sets. Five biomarkers discovered in the sera from experimentally infected pigs were identified as clusterin, lecithin-cholesterol acyltransferase, vitronectin, haptoglobin and apolipoprotein A-I.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Apolipoprotein A-I / blood
  • Biomarkers / blood*
  • Chromatography, Liquid
  • Clusterin / blood
  • Cysticercosis / blood
  • Cysticercosis / diagnosis
  • Cysticercosis / veterinary*
  • Female
  • Haptoglobins / analysis
  • Male
  • Peru
  • Phosphatidylcholine-Sterol O-Acyltransferase / blood
  • Protein Array Analysis*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Swine
  • Swine Diseases / blood
  • Swine Diseases / diagnosis*
  • Swine Diseases / parasitology
  • Taenia solium
  • Tandem Mass Spectrometry
  • Vitronectin / blood
  • Zambia


  • Apolipoprotein A-I
  • Biomarkers
  • Clusterin
  • Haptoglobins
  • Vitronectin
  • Phosphatidylcholine-Sterol O-Acyltransferase