Objective: To investigate the effects of Rubus suavissimus S. Lee extract (RSE) on mice, and its influence on release of histamine from rat peritoneal mast cells induced by compound 48/80 in vitro.
Methods: To establish murine delayed-type-hypersensitive model induced by 2,4-dinitrofluotobenzene (DNFB) and sheep red blood cell (SRBC); murine type I allergic reaction induced by PCA, and then to observe the anti-allergic effect of RSE. HPLC-ECD methods were performed to detect the contents of histamine released from rat peritoneal mast cells induced by compound 48/80 in vitro.
Results: Compared with the model group, RSE could ameliorate the ear swelling and capillary permeability in mice induced by DNFB and PCA. RSE also reduced the thickness of the mice hind paw induced by SRBC in significant dose-dependent manner. Meanwhile, RSE obviously inhibited the release of histamine in rat peritoneal mast in vitro, a dose-dependent manner.
Conclusion: RSE exerts potential anti-allergy effects and the mechanisms may be partly related to its inhibitory effect on the release of histamine from mast cells.