Isolation and characterization of the CRIPTO autosomal gene and its X-linked related sequence

Am J Hum Genet. 1991 Sep;49(3):555-65.


We have previously reported on the identification of a cDNA clone encoding a novel human growth factor, named "CRIPTO," that is abundantly expressed in undifferentiated human NTERA-2 clone D1 (NT2/D1) and mouse (F9) teratocarcinoma cells. We now report the organization and nucleotide sequence of two related genomic sequences. One (CR-1) corresponds to the structural gene encoding the human CRIPTO protein expressed in the undifferentiated human teratocarcinoma cells, and the other (CR-3) corresponds to a complete copy of the mRNA containing seven base substitutions in the coding region representing both silent and replacement substitutions. The 440 bp 5' to the CAP site of CR-1 are preserved in CR-3. CR-1 maps to chromosome 3, and CR-3 maps to Xq21-q22. Southern blot analysis reveals that multiple CRIPTO-related DNA sequences are present in the human as well as in the mouse genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 3*
  • Epidermal Growth Factor*
  • GPI-Linked Proteins
  • Growth Substances / genetics*
  • Humans
  • Intercellular Signaling Peptides and Proteins
  • Membrane Glycoproteins*
  • Mice
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Mutation / genetics
  • Neoplasm Proteins / genetics*
  • Tumor Cells, Cultured
  • X Chromosome*


  • GPI-Linked Proteins
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Membrane Glycoproteins
  • Neoplasm Proteins
  • TDGF1 protein, human
  • Tdgf1 protein, mouse
  • Epidermal Growth Factor

Associated data

  • GENBANK/M77181
  • GENBANK/M96955
  • GENBANK/M96956
  • GENBANK/S55792
  • GENBANK/S62061
  • GENBANK/S62063
  • GENBANK/S62066
  • GENBANK/S62712
  • GENBANK/S68601
  • GENBANK/S68604