Role of immunoexpression of nitric oxide synthases by Hodgkin and Reed-Sternberg cells on apoptosis deregulation and on clinical outcome of classical Hodgkin lymphoma

Mol Cell Biochem. 2009 Jan;321(1-2):95-102. doi: 10.1007/s11010-008-9923-1. Epub 2008 Oct 2.


Hodgkin and Reed-Sternberg (H-RS) cells of classical Hodgkin lymphoma (cHL) present an impaired expression of immunoglobulin genes, but escape apoptotic death. We investigated whether nitric oxide synthases (NOS) are expressed by H-RS cells, studied their association with EBV status and the expression of apoptotic proteins, and investigated their relationship to the clinical outcome of 171 patients. NOS1 and NOS2 were expressed in a large number of cases, whereas NOS3 expression was not detected. Positive associations were found between NOS1 and p53, bax and NOS2, bcl-2 and NOS2, bax and p53, and between bax and fasL. Inverse correlations were established between EBV and NOS2 and between EBV and bcl-2. A shorter overall survival (OS) was associated with strong expression of NOS2. In conclusion, NOS are expressed by H-RS cells of cHL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Apoptosis / immunology*
  • Child
  • Child, Preschool
  • Female
  • Hodgkin Disease / immunology*
  • Hodgkin Disease / physiopathology
  • Humans
  • Isoenzymes / metabolism*
  • Male
  • Middle Aged
  • Nitric Oxide Synthase / metabolism*
  • Reed-Sternberg Cells / cytology
  • Reed-Sternberg Cells / enzymology*
  • Reed-Sternberg Cells / immunology*
  • Retrospective Studies
  • Survival Analysis
  • Young Adult


  • Isoenzymes
  • Nitric Oxide Synthase