Molecular architecture of the "stressosome," a signal integration and transduction hub

Science. 2008 Oct 3;322(5898):92-6. doi: 10.1126/science.1159572.

Abstract

A commonly used strategy by microorganisms to survive multiple stresses involves a signal transduction cascade that increases the expression of stress-responsive genes. Stress signals can be integrated by a multiprotein signaling hub that responds to various signals to effect a single outcome. We obtained a medium-resolution cryo-electron microscopy reconstruction of the 1.8-megadalton "stressosome" from Bacillus subtilis. Fitting known crystal structures of components into this reconstruction gave a pseudoatomic structure, which had a virus capsid-like core with sensory extensions. We suggest that the different sensory extensions respond to different signals, whereas the conserved domains in the core integrate the varied signals. The architecture of the stressosome provides the potential for cooperativity, suggesting that the response could be tuned dependent on the magnitude of chemophysical insult.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacillus subtilis / chemistry*
  • Bacillus subtilis / metabolism
  • Bacillus subtilis / ultrastructure
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism
  • Bacterial Proteins / ultrastructure
  • Cryoelectron Microscopy
  • Crystallography, X-Ray
  • Image Processing, Computer-Assisted
  • Models, Biological
  • Models, Molecular
  • Molecular Sequence Data
  • Multiprotein Complexes / chemistry*
  • Multiprotein Complexes / metabolism
  • Multiprotein Complexes / ultrastructure
  • Phosphoproteins / chemistry*
  • Phosphoproteins / metabolism
  • Phosphoproteins / ultrastructure
  • Phosphorylation
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases / chemistry*
  • Protein-Serine-Threonine Kinases / metabolism
  • Protein-Serine-Threonine Kinases / ultrastructure
  • Sigma Factor / metabolism
  • Signal Transduction*

Substances

  • Bacterial Proteins
  • Multiprotein Complexes
  • Phosphoproteins
  • RsbR protein, Bacillus subtilis
  • Sigma Factor
  • RsbT protein, Bacillus subtilis
  • Protein-Serine-Threonine Kinases

Associated data

  • OMIM/2VY9