Development and evaluation of a rapid absorbed enzyme immunoassay test for the diagnosis of Johne's disease in cattle

Aust Vet J. 1991 May;68(5):157-60. doi: 10.1111/j.1751-0813.1991.tb03167.x.


An absorbed enzyme immunoassay (EIA) test for Johne's disease in cattle was developed in which absorption of cross-reacting antibodies occurred as a rapid reaction in solution rather than overnight with whole organisms and a subsequent centrifugation step. Total test time was reduced to less than 2 h with a minimum of manipulations. The test was evaluated in cattle herds from Johne's disease-endemic and Johne's disease-free regions of Australia. Specificity was 99.8%. Calculations of sensitivity were affected by the history of the herd under test. However, the EIA detected in excess of 80% of animals before onset of clinical disease and 65% of faecal shedders were EIA positive on, or before, first detection of Mycobacterium paratuberculosis in their faeces. The test should aid epidemiological studies and be a useful tool in the management and control of Johne's disease.

MeSH terms

  • Absorption
  • Animals
  • Antibodies, Bacterial / blood*
  • Antigens, Bacterial / immunology*
  • Cattle
  • Evaluation Studies as Topic
  • Feces / microbiology
  • Immune Sera / immunology
  • Immunoenzyme Techniques*
  • Mycobacterium / immunology*
  • Mycobacterium / isolation & purification
  • Mycobacterium avium / immunology
  • Paratuberculosis / diagnosis*
  • Paratuberculosis / microbiology
  • Predictive Value of Tests
  • Reagent Kits, Diagnostic / veterinary


  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Immune Sera
  • Reagent Kits, Diagnostic