Postnatal catch-up growth after fetal protein restriction programs proliferation of rat preadipocytes

Obesity (Silver Spring). 2008 Dec;16(12):2760-3. doi: 10.1038/oby.2008.417. Epub 2008 Oct 2.


We studied the in vitro proliferation and differentiation of rat preadipocytes to investigate whether catch-up growth after prenatal protein restriction may program adipose precursor cells leading to development of increased adipose tissue mass. Pregnant rat dams were fed either an isocaloric low-protein diet (LP-8%) or control diet (C-20%). During lactation, in order to induce catch-up growth, dams from LP group were fed with the C diet and litter size was reduced to four pups instead of eight. Preadipocytes were isolated from weanling male pups (28 days of age). Differentiation and proliferation were assessed across time. At late stages of preadipocyte differentiation, no difference was observed in lipid accumulation of C or LP cultures but the mRNA expression of leptin was enhanced in LP cells. At early stages of culture, a higher DNA and protein content accompanied by a higher rate of proliferation was measured in adipocytes from LP cultures. Moreover, the mRNA expression of cyclin D1 was increased in these cells whereas the expression of peroxisome proliferators-activated receptor gamma (PPARgamma) and steroyl regulatory element binding protein (SREBP-1c) was significantly reduced during early stages. The results suggest that prenatal exposure to a LP followed by rapid catch-up growth is associated with a higher rate for proliferation in preadipocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology*
  • Adipocytes / metabolism
  • Adipose Tissue / growth & development*
  • Animals
  • Animals, Newborn
  • Cell Differentiation*
  • Cell Proliferation*
  • Cyclin D1 / metabolism
  • Diet, Protein-Restricted*
  • Female
  • Growth*
  • Leptin / metabolism
  • Male
  • PPAR gamma / metabolism
  • Pregnancy
  • Proteins / analysis
  • RNA, Messenger / metabolism
  • Rats
  • Sterol Regulatory Element Binding Protein 1 / metabolism


  • Ccnd1 protein, rat
  • Leptin
  • PPAR gamma
  • Proteins
  • RNA, Messenger
  • Srebf1 protein, rat
  • Sterol Regulatory Element Binding Protein 1
  • Cyclin D1