Complementary DNA sequence of rabbit CAP18--a unique lipopolysaccharide binding protein

Biochem Biophys Res Commun. 1991 Aug 30;179(1):170-5. doi: 10.1016/0006-291x(91)91350-l.

Abstract

CAP18 is a novel 18 kDa cationic protein [pI approximately 10] originally purified from rabbit granulocytes using as an assay the agglutination of lipopolysaccharide (LPS) coated erythrocytes. cDNA clones encoding CAP18 were isolated from a rabbit bone marrow cDNA library using a PCR generated oligonucleotide probe derived from the N-terminal amino acid sequence. The deduced amino acid sequence reveals a putative signal sequence of 29 amino acids and a mature protein of 142 amino acid residues. The predicted size of the encoded protein is 16.6 kDa with a pI of 10. There are no N-linked glycosylation sites. The CAP18 sequence bears no homology with other known LPS-binding proteins including human bacterial permeability increasing protein (BPI)(1) and rabbit LPS binding protein (LBP)(2).

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antimicrobial Cationic Peptides*
  • Base Sequence
  • Blotting, Northern
  • Bone Marrow / physiology
  • Carrier Proteins / genetics*
  • Cathelicidins
  • Cloning, Molecular
  • DNA / genetics*
  • DNA / isolation & purification
  • Gene Library
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Rabbits

Substances

  • Antimicrobial Cationic Peptides
  • Carrier Proteins
  • Cathelicidins
  • Oligonucleotide Probes
  • RNA, Messenger
  • CAP18 lipopolysaccharide-binding protein
  • DNA

Associated data

  • GENBANK/M73998
  • GENBANK/M74785
  • GENBANK/M74786
  • GENBANK/M74787
  • GENBANK/M74788
  • GENBANK/M74790
  • GENBANK/M81108
  • GENBANK/M81109
  • GENBANK/S57525
  • GENBANK/S57567