Transmembrane signalling by the N-formyl peptide receptor in stably transfected fibroblasts

Biochem Biophys Res Commun. 1991 Aug 30;179(1):471-6. doi: 10.1016/0006-291x(91)91394-r.


We investigated the requirement for N-formyl peptide receptor-mediated transmembrane signalling in transfected mouse fibroblasts that express the receptor. Stably transfected cells displayed specific binding for N-formyl-Met-Leu-[3H]Phe with a dissociation constant of 3 nM. The cells responded to ligand stimulation with mobilization of calcium from intracellular stores. Calcium mobilization was ligand dose-dependent (EC50 = 3 nM fMet-Leu-Phe) and could be inhibited by pertussis toxin treatment. These results provide the first demonstration that expression of the single-chain N-formyl peptide receptor in mouse fibroblasts is sufficient for mediating ligand-induced early transmembrane signalling events, which do not appear to require other neutrophil-specific cellular components.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Binding, Competitive
  • Calcium / metabolism
  • Cell Line
  • Cell Membrane / metabolism
  • Cell Membrane / physiology
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Humans
  • Kinetics
  • Mice
  • Molecular Sequence Data
  • N-Formylmethionine Leucyl-Phenylalanine / metabolism*
  • Oligonucleotide Probes
  • Pertussis Toxin
  • Polymerase Chain Reaction
  • Receptors, Formyl Peptide
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / physiology*
  • Signal Transduction*
  • Transfection*
  • Virulence Factors, Bordetella / pharmacology


  • Oligonucleotide Probes
  • Receptors, Formyl Peptide
  • Receptors, Immunologic
  • Virulence Factors, Bordetella
  • N-Formylmethionine Leucyl-Phenylalanine
  • Pertussis Toxin
  • Calcium