Clinical application of in situ tissue engineering using a scaffolding technique for reconstruction of the larynx and trachea

Ann Otol Rhinol Laryngol. 2008 Sep;117(9):673-8. doi: 10.1177/000348940811700908.


Objectives: The objective of the present study was to demonstrate the efficacy of the clinical application of in situ tissue engineering using a scaffolding technique for laryngeal and tracheal tissue.

Methods: We have developed a tissue scaffold made from a Marlex mesh tube covered by collagen sponge. Based on successful animal experimental studies, in situ tissue engineering with a scaffold implant was applied to repair the larynx and trachea in 4 patients.

Results: In 1 patient with subglottic stenosis, the thyroid cartilage, cricoid cartilage, and cervical trachea with scarring and granulation were resected and reconstructed by use of the scaffold. In 3 patients with thyroid cancer, the trachea and cricoid cartilage with tumor invasion were resected and the scaffold was implanted into the defect. Postoperative endoscopy during the observation period of 8 to 34 months showed a well-epithelialized airway lumen without any obstruction.

Conclusions: Our current technique of in situ tissue engineering using a scaffold shows great potential for use in the regeneration of airway defects.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Cricoid Cartilage / surgery
  • Female
  • Humans
  • Laryngostenosis / surgery
  • Larynx / surgery*
  • Male
  • Middle Aged
  • Plastic Surgery Procedures / methods
  • Thyroid Cartilage / surgery
  • Thyroid Neoplasms / surgery
  • Tissue Engineering / methods*
  • Tissue Scaffolds*
  • Trachea / surgery*