High-speed, miniaturized fluorescence microscopy in freely moving mice

Nat Methods. 2008 Nov;5(11):935-8. doi: 10.1038/nmeth.1256. Epub 2008 Oct 5.


A central goal in biomedicine is to explain organismic behavior in terms of causal cellular processes. However, concurrent observation of mammalian behavior and underlying cellular dynamics has been a longstanding challenge. We describe a miniaturized (1.1 g mass) epifluorescence microscope for cellular-level brain imaging in freely moving mice, and its application to imaging microcirculation and neuronal Ca(2+) dynamics.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Brain / physiology
  • Fiber Optic Technology / instrumentation
  • Fiber Optic Technology / methods
  • Mice
  • Microscopy, Fluorescence / instrumentation*
  • Microscopy, Fluorescence / methods*
  • Miniaturization / instrumentation
  • Miniaturization / methods*
  • Movement / physiology*
  • Time Factors