Abstract
A central goal in biomedicine is to explain organismic behavior in terms of causal cellular processes. However, concurrent observation of mammalian behavior and underlying cellular dynamics has been a longstanding challenge. We describe a miniaturized (1.1 g mass) epifluorescence microscope for cellular-level brain imaging in freely moving mice, and its application to imaging microcirculation and neuronal Ca(2+) dynamics.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Brain / physiology
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Fiber Optic Technology / instrumentation
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Fiber Optic Technology / methods
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Mice
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Microscopy, Fluorescence / instrumentation*
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Microscopy, Fluorescence / methods*
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Miniaturization / instrumentation
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Miniaturization / methods*
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Movement / physiology*
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Time Factors