In college and adult samples, women score higher then men on the Five Factor Model (FFM) personality traits of Neuroticism and Agreeableness. The present study assessed the extent to which these gender differences held in a sample of 486 older adults, ranging in age from 65-98 (M = 75, SD = 6.5), using the NEO-Five Factor Inventory. Mean and Covariance Structure models testing gender differences at the level of latent traits revealed higher levels of Neuroticism (d = .52) and Agreeableness (d = .35) in older women than older men. The consistency of these findings with prior work in younger samples attests to the stability of gender differentiation on Neuroticism and Agreeableness across the lifespan. Gender differences on these traits should be considered in personality research among older, as well as middle age and younger adults.