[Effects of Rehmannia root decoction serum on cell proliferation and EPO expression in cultured human umbilical vein endothelial cells]

Zhongguo Zhong Yao Za Zhi. 2008 Jul;33(13):1579-82.
[Article in Chinese]


Objective: To explore the efficacy of the rehmannia root decoction containing serum in enhencing the proliferation of HUVECs-1 and EPO expression and to give libratory evidence for the tonifying blood and kidney therapy with rehmannia root.

Method: Twenty-four male Sprague-Dawley rats were randomly devided into 4 group and administrered by gastrogavage rehmannia root decoction of 3, 6, 10 g x kg(-1) once a day for 1 weeks. Using serum pharmacologic method, the proliferation of HUVECs-1 cell was determined by MTT chromatometry at 48 h points by co-culturing with medicated serum containing different concentration of rehmannia root decoction. The expression of EPO on HUVECs-1 were observed by immunity cytochemistry and Western blot. NS serum was taken as control.

Result: After the HUVECs-1 cultivating with medicated serum for 48 h, compared with NS serum, 20% serum containing rehmannia root (6 g x kg(-1)) could obviously increase proliferation of HUVEC-1 (P < 0.05) and increase the level of the expression of EPO on HUVECs-1 (P < 0.05).

Conclusion: Rehmannia root extract has good actions of proliferation of HUVECs-1 and increase EPO expression, which is the mechanisms of nourishing yin and blood and tonifying essence of rehmannia root.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Drugs, Chinese Herbal / administration & dosage
  • Drugs, Chinese Herbal / pharmacology*
  • Endothelial Cells / cytology*
  • Endothelial Cells / drug effects
  • Endothelial Cells / metabolism*
  • Erythropoietin / metabolism*
  • Gene Expression Regulation / drug effects*
  • Humans
  • Male
  • Plant Roots / chemistry*
  • Rats
  • Rats, Sprague-Dawley
  • Rehmannia / chemistry*
  • Serum / chemistry
  • Umbilical Veins / cytology*


  • Drugs, Chinese Herbal
  • Erythropoietin