Abstract
A cDNA for mRNA induced by antimycin A in Hansenula anomala was cloned. The mRNA for the cDNA was expressed in the yeast under the conditions expressing the cyanide-resistant respiration activity. The nucleotide sequence revealed a long open reading frame of 342 codons encoding a protein with a molecular weight of 40,282 in the cDNA. An antibody recognizing the protein encoded by the open reading frame was produced. Immunoblotting of H. anomala proteins with this antibody showed that a 36 kDa protein localized in mitochondria was a mature form of the protein encoded by the cDNA. It is suggested that the cloned cDNA encodes a protein involved in the cyanide-resistant respiratory pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antimycin A / pharmacology*
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Base Sequence
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Cloning, Molecular
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Cyanides / pharmacology
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Fungal Proteins / chemistry
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Fungal Proteins / genetics*
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Gene Expression Regulation, Fungal / drug effects*
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Immunoblotting
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Mitochondria / metabolism
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Molecular Sequence Data
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Open Reading Frames / genetics
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Oxidoreductases / genetics
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Oxygen Consumption
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Pichia / genetics*
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Pichia / metabolism
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RNA, Messenger / genetics*
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Restriction Mapping
Substances
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Cyanides
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Fungal Proteins
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RNA, Messenger
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Antimycin A
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Oxidoreductases
Associated data
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GENBANK/D00741
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GENBANK/D00760
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GENBANK/D00761
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GENBANK/D00762
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GENBANK/D00763
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GENBANK/M75974
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GENBANK/M75975
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GENBANK/S55723
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GENBANK/X57933
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GENBANK/X57934
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GENBANK/X57935