Molecular cloning of cDNA for antimycin A-inducible mRNA and its role in cyanide-resistant respiration in Hansenula anomala

Biochim Biophys Acta. 1991 Aug 27;1090(1):102-8. doi: 10.1016/0167-4781(91)90043-l.


A cDNA for mRNA induced by antimycin A in Hansenula anomala was cloned. The mRNA for the cDNA was expressed in the yeast under the conditions expressing the cyanide-resistant respiration activity. The nucleotide sequence revealed a long open reading frame of 342 codons encoding a protein with a molecular weight of 40,282 in the cDNA. An antibody recognizing the protein encoded by the open reading frame was produced. Immunoblotting of H. anomala proteins with this antibody showed that a 36 kDa protein localized in mitochondria was a mature form of the protein encoded by the cDNA. It is suggested that the cloned cDNA encodes a protein involved in the cyanide-resistant respiratory pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antimycin A / pharmacology*
  • Base Sequence
  • Cloning, Molecular
  • Cyanides / pharmacology
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • Gene Expression Regulation, Fungal / drug effects*
  • Immunoblotting
  • Mitochondria / metabolism
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Oxidoreductases / genetics
  • Oxygen Consumption
  • Pichia / genetics*
  • Pichia / metabolism
  • RNA, Messenger / genetics*
  • Restriction Mapping


  • Cyanides
  • Fungal Proteins
  • RNA, Messenger
  • Antimycin A
  • Oxidoreductases

Associated data

  • GENBANK/D00741
  • GENBANK/D00760
  • GENBANK/D00761
  • GENBANK/D00762
  • GENBANK/D00763
  • GENBANK/M75974
  • GENBANK/M75975
  • GENBANK/S55723
  • GENBANK/X57933
  • GENBANK/X57934
  • GENBANK/X57935