Chemotactic potency of recombinant human neutrophil attractant/activation protein-1 (interleukin-8) for polymorphonuclear leukocytes of different species

Cytokine. 1991 Jan;3(1):21-7. doi: 10.1016/1043-4666(91)90006-y.


In order to establish the species cross-reactivity of the human neutrophil attractant/activation protein-1 (interleukin-8, NAP-1/IL-8) and find which experimental species are responsive to the human cytokine, blood polymorphonuclear leukocytes (PNMLs) were isolated from chicken, dog, goat, guinea-pig, monkey, mouse, pig, rabbit, and rat and their in vitro migration in response to this cytokine was investigated. PMNLs from all of the tested species migrated in response to recombinant human NAP-1/IL-8 (rhNAP-1/IL-8). The potency of rhNAP-1/IL-8 for the PMNLs of different species varied and was considerably lower than its potency for human cells. The morphological study combined with the leukocyte enumeration in the intradermal rhNAP-1/IL-8 injection sites established an in vivo proinflammatory potency of rhNAP-1/IL-8 for rabbit and rat that was comparable to the observed in vitro chemotactic potency of rhNAP-1/IL-8 for neutrophils of these species.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Chemotaxis, Leukocyte / drug effects*
  • Dose-Response Relationship, Drug
  • Humans
  • In Vitro Techniques
  • Interleukin-8 / pharmacology*
  • Neutrophils / drug effects
  • Neutrophils / physiology*
  • Recombinant Proteins / pharmacology
  • Species Specificity


  • Interleukin-8
  • Recombinant Proteins