Establishment of a humanized model of liver using NOD/Shi-scid IL2Rgnull mice

Biochem Biophys Res Commun. 2008 Dec 5;377(1):248-52. doi: 10.1016/j.bbrc.2008.09.124. Epub 2008 Oct 7.


Severely immunodeficient NOD/Shi-scid IL2Rg(null) (NOG) mice are used as recipients for human tissue transplantation, which produces chimeric mice with various types of human tissue. NOG mice expressing transgenic urokinase-type plasminogen activator in the liver (uPA-NOG) were produced. Human hepatocytes injected into uPA-NOG mice repopulated the recipient livers with human cells. The uPA-NOG model has several advantages over previously produced chimeric mouse models of human liver: (1) the severely immunodeficient NOG background enables higher xenogeneic cell engraftment; (2) the absence of neonatal lethality enables mating of homozygotes, which increased the efficacy of homozygote production; and (3) donor xenogeneic human hepatocytes could be readily transplanted into young uPA-NOG mice, which provide easier surgical manipulation and improved recipient survival.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Hepatocytes / metabolism
  • Hepatocytes / transplantation
  • Humans
  • Interleukin-2 Receptor alpha Subunit / genetics
  • Liver / cytology
  • Liver / metabolism*
  • Liver Transplantation
  • Mice
  • Mice, Transgenic*
  • Models, Animal*
  • Pharmaceutical Preparations / metabolism
  • Stem Cells / cytology
  • Transplantation, Heterologous
  • Urokinase-Type Plasminogen Activator / genetics


  • Il2ra protein, mouse
  • Interleukin-2 Receptor alpha Subunit
  • Pharmaceutical Preparations
  • Urokinase-Type Plasminogen Activator