Increased 8-isoprostane, a marker of oxidative stress in exhaled breath condensate in subjects with asbestos exposure

Ind Health. 2008 Oct;46(5):484-9. doi: 10.2486/indhealth.46.484.


Asbestosis and pleural plaques exhibit unpredictable but progressive development, and there are no markers routinely available to measure their prognosis. Asbestos exposure induces the generation of reactive oxygen species, and 8-isoprostane is involved in experimental asbestos-related lung toxicity. This oxidative stress marker was measured in exhaled breath condensate (EBC) in 92 former asbestos workers with mean age 68.8+/-1.7 yr and mean duration of asbestos exposure 24.1+/-2.0 yr. The control group had 46 subjects with mean age 65.2+/-3.3 yr. The mean level of 8-isoprostane, analyzed by liquid chromatography-electrospray ionization-mass spectrometry, was higher in asbestos-exposed subjects (69.5+/-6.6 pg/ml, p=0.0001) compared with the control group, where the concentration was 47.0+/-7.8 pg/ml. The results presented support the hypothesis that oxidative stress due to asbestos is the main cause of increased 8-isoprostane in EBC. Measurement of 8-isoprostane in EBC is a promising non-invasive means for assessing the activity of asbestos-induced diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Asbestos / metabolism*
  • Asbestos / poisoning*
  • Asbestosis / metabolism*
  • Biomarkers / analysis
  • Breath Tests / methods
  • Case-Control Studies
  • Dinoprost / analogs & derivatives*
  • Dinoprost / analysis
  • Dinoprost / metabolism
  • Female
  • Humans
  • Male
  • Middle Aged
  • Occupational Exposure
  • Oxidative Stress
  • Prognosis
  • Risk Assessment


  • Biomarkers
  • Asbestos
  • 8-epi-prostaglandin F2alpha
  • Dinoprost