Kineococcus gynurae sp. nov., isolated from a Thai medicinal plant

Int J Syst Evol Microbiol. 2008 Oct;58(Pt 10):2439-42. doi: 10.1099/ijs.0.65671-0.

Abstract

A novel, Gram-positive, motile, coccus-shaped, orange-pigmented organism, designated strain KKD096(T), was isolated from the roots of a Thai medicinal plant, Gynura pseudochina DC. var. hispida Thwaites. Growth of strain KKD096(T) occurred at temperatures of 14-34 degrees C, at pH 5.0-9.0 and at NaCl concentrations up to 7 % (w/v). Whole-cell hydrolysates contained arabinose and galactose as the characteristic sugars. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The glycan moiety of the murein contained acetyl residues. The predominant menaquinone was MK-9(H2); mycolic acids were not detected. The genomic DNA G+C content was 73.3 mol%. The major cellular fatty acid was anteiso-C(15 : 0) (81.42 % of the total). Strain KKD096(T) was assigned to the genus Kineococcus on the basis of 16S rRNA gene sequence analysis; it was most closely related to Kineococcus radiotolerans DSM 14245(T) (97.1 % similarity). DNA-DNA hybridization revealed 39.4 % relatedness between these two taxa. On the basis of the genotypic and phenotypic data presented, strain KKD096(T) is considered to represent a novel species of the genus Kineococcus, for which the name Kineococcus gynurae sp. nov. is proposed. The type strain is KKD096(T) (=TISTR 1856(T)=NRRL B-24568(T)=BCC 26245(T)=NBRC 103943(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / chemistry
  • Actinomycetales / classification*
  • Actinomycetales / genetics*
  • Actinomycetales / isolation & purification
  • Asteraceae / microbiology*
  • Base Composition
  • DNA, Bacterial / genetics
  • Diaminopimelic Acid / chemistry
  • Fatty Acids / chemistry
  • Genes, Bacterial
  • Genes, rRNA
  • Genotype
  • Molecular Sequence Data
  • Peptidoglycan / chemistry
  • Phenotype
  • Phylogeny
  • Plant Roots / microbiology*
  • Plants, Medicinal / microbiology
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Thailand
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Peptidoglycan
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 9
  • Diaminopimelic Acid

Associated data

  • GENBANK/EF667339