Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase

Genes Dev. 1991 Sep;5(9):1589-600. doi: 10.1101/gad.5.9.1589.

Abstract

Replication factor-A (RF-A) is a three-subunit protein complex originally purified from human cells as an essential component for SV40 DNA replication in vitro. We have previously identified a functionally homologous three-subunit protein complex from the yeast Saccharomyces cerevisiae. Here we report the cloning and characterization of the genes encoding RF-A from S. cerevisiae. Each of the three subunits is encoded by a single essential gene. Cells carrying null mutations in any of the three genes arrest as budded and multiply budded cells. All three genes are expressed in a cell-cycle-dependent manner; the mRNA for each subunit peaks at the G1/S-phase boundary. A comparison of protein sequences indicates that the human p34 subunit is 29% identical to the corresponding RFA2 gene product. However, expression of the human protein fails to rescue the rfa2::TRP1 disruption.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA Replication / genetics*
  • DNA, Fungal
  • DNA-Binding Proteins / genetics*
  • Fungal Proteins / genetics*
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Humans
  • Introns
  • Molecular Sequence Data
  • Phenotype
  • Plasmids
  • Replication Protein A
  • Restriction Mapping
  • S Phase
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Sequence Alignment

Substances

  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • RPA1 protein, human
  • Replication Protein A

Associated data

  • GENBANK/X59748
  • GENBANK/X59749
  • GENBANK/X59750