The Histone Gene Cell Cycle Regulator HiNF-P Is a Unique Zinc Finger Transcription Factor With a Novel Conserved Auxiliary DNA-binding Motif

Biochemistry. 2008 Nov 4;47(44):11415-23. doi: 10.1021/bi800961d. Epub 2008 Oct 14.


Accumulation of histone proteins is necessary for packaging of replicated DNA during the S phase of the cell cycle. Different mechanisms operate to regulate histone protein levels, and induction of human histone gene expression at the G1-S phase transition plays a critical role. The zinc finger HiNF-P and coactivator p220 (NPAT) proteins are key regulators of histone gene expression. Here, we describe a novel HiNF-P-specific conserved region (PSCR) located within the C-terminus that is present in HiNF-P homologues of all metazoan species that have been examined. The PSCR motif is required for activation of histone H4 gene transcription and contributes to DNA binding of HiNF-P. Thus, the PSCR module represents an auxiliary DNA-binding determinant that plays a critical role in mediating histone gene expression during the cell cycle and defines HiNF-P as a unique cell cycle regulatory member of the zinc finger transcription factor family.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites
  • Cell Cycle
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • Cell Line
  • Conserved Sequence
  • DNA / genetics
  • DNA / metabolism
  • HeLa Cells
  • Histones / genetics
  • Humans
  • Models, Molecular
  • Molecular Sequence Data
  • Multiprotein Complexes
  • Mutagenesis
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • RNA, Small Interfering / genetics
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Repressor Proteins / chemistry*
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Sequence Homology, Amino Acid
  • Zinc Fingers


  • Cell Cycle Proteins
  • HINFP protein, human
  • Histones
  • Multiprotein Complexes
  • NPAT protein, human
  • Nuclear Proteins
  • RNA, Small Interfering
  • Recombinant Proteins
  • Repressor Proteins
  • DNA