Soluble CD40 ligand as a biomarker for storage-related preanalytic variations of human serum

Cytokine. 2008 Nov;44(2):275-82. doi: 10.1016/j.cyto.2008.08.010. Epub 2008 Oct 11.


There are currently no appropriate and sensitive biomarkers available to assess preanalytic variations in human biological fluids stored in biobanks. We identified soluble CD40 ligand (sCD40L) as the first ubiquitous biomarker to show an on-off response in serum exposed to moderate or elevated room temperature conditions. We used immunoenzyme assays to monitor the sCD40L response after 12 h storage at 37 degrees C or 48 h at 20 degrees C. Our findings show that prolonged storage of serum samples at elevated room temperature can be determined by the absence of detectable sCD40L.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Biological Specimen Banks*
  • Biomarkers / blood*
  • Blood Preservation*
  • Blood Specimen Collection / methods
  • Body Fluids / chemistry
  • CD40 Ligand / blood*
  • Cytokines / blood
  • Female
  • Humans
  • Male
  • Middle Aged
  • Quality Control
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Serum* / chemistry


  • Biomarkers
  • Cytokines
  • CD40 Ligand