Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells

Biochem Biophys Res Commun. 2008 Dec 12;377(2):458-462. doi: 10.1016/j.bbrc.2008.09.154. Epub 2008 Oct 11.


The objective of this study was to investigate the influence of dynamic compressive loading on chondrogenesis of mesenchymal stem cells (MSCs) in the presence of TGF-beta3. Isolated porcine MSCs were suspended in 2% agarose and subjected to intermittent dynamic compression (10% strain) for a period of 42 days in a dynamic compression bioreactor. After 42 days in culture, the free-swelling specimens exhibited more intense alcian blue staining for proteoglycans, while immunohistochemical analysis revealed increased collagen type II immunoreactivity. Glycosaminoglycan (GAG) content increased with time for both free-swelling and dynamically loaded constructs, and by day 42 it was significantly higher in both the core (2.5+/-0.21%w/w vs. 0.94+/-0.03%w/w) and annulus (1.09+/-0.09%w/w vs. 0.59+/-0.08%w/w) of free-swelling constructs compared to dynamically loaded constructs. This result suggests that further optimization is required in controlling the biomechanical and/or the biochemical environment if such stimuli are to have beneficial effects in generating functional cartilaginous tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bioreactors
  • Cell Culture Techniques
  • Chondrogenesis* / drug effects
  • Chondrogenesis* / physiology
  • Collagen Type II / metabolism
  • Compressive Strength*
  • Glycosaminoglycans / metabolism
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / physiology
  • Swine
  • Transforming Growth Factor beta3 / pharmacology


  • Collagen Type II
  • Glycosaminoglycans
  • Transforming Growth Factor beta3