Inhibition of production and function of interleukin-6 by 1,25-dihydroxyvitamin D3

Immunol Lett. 1991 May;28(2):115-20. doi: 10.1016/0165-2478(91)90108-m.


1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) has been shown to interfere with immunoglobulin production and lymphocyte proliferation in vitro. These lymphocyte functions are influenced by interleukin (IL)-6 produced by antigen presenting cells. Hence, the ability of 1,25-(OH)2D3 to interfere with the production and function of IL-6 was investigated. 1,25-(OH)2D3 and the analogue MC 903 inhibited IL-6 production by LPS-stimulated human mononuclear cells. The precursor 25-OH D3 was ineffective. Likewise, 1,25-(OH)2D3 but not 25-OH D3 inhibited rIL-6-driven as well as rIL-1 alpha/beta-driven proliferation of murine thymocytes. This effect of 1,25-(OH)2D3 was partially or totally overcome by larger concentrations of rIL-6 as well as by rIL-2 and ionomycin. Consistently, the production of IL-6 and IL-2 in rIL-1 driven thymocyte cultures were found to be reduced by 1,25-(OH)2D3. Inhibition of production and function of IL-6 may therefore be involved in 1,25-(OH)2D3-mediated regulation of lymphocyte functions in vitro.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcifediol / pharmacology
  • Calcitriol / analogs & derivatives
  • Calcitriol / pharmacology*
  • Cells, Cultured
  • Depression, Chemical
  • Humans
  • Interleukin-2 / biosynthesis
  • Interleukin-6 / antagonists & inhibitors*
  • Interleukin-6 / biosynthesis
  • Leukocytes, Mononuclear / drug effects
  • Lymphocyte Activation / drug effects
  • Mice
  • Mice, Inbred C3H
  • Thymus Gland / cytology
  • Thymus Gland / drug effects


  • Interleukin-2
  • Interleukin-6
  • calcipotriene
  • Calcitriol
  • Calcifediol