Identification of a 200-kDa glycoprotein antigen of Saccharomyces cerevisiae

Immunol Lett. 1991 Jun;28(3):181-5. doi: 10.1016/0165-2478(91)90001-q.

Abstract

SDS-PAGE analysis of the soluble proteins of the yeast Saccharomyces cerevisiae revealed a single predominant moiety of apparent size 200 kDa which was strongly periodic acid Schiff-positive. In immunoblotting experiments, human sera containing anti-S. cerevisiae antibodies were reactive only with this glycoprotein (gp200); this was demonstrated using sera containing IgG and IgA isotype-specific anti-S. cerevisiae antibodies. The antigenicity of gp200 was substantially reduced by periodate treatment. These results show that gp200 is a major glycoprotein antigen of S. cerevisiae which is immunogenic in man.

MeSH terms

  • Antibodies, Fungal / immunology
  • Antigens, Fungal / isolation & purification*
  • Crohn Disease / immunology
  • Cross Reactions / immunology
  • Electrophoresis, Polyacrylamide Gel
  • Glycoproteins / isolation & purification*
  • Humans
  • Immunoblotting
  • Immunoglobulin A / immunology
  • Immunoglobulin G / immunology
  • Molecular Weight
  • Saccharomyces cerevisiae / immunology*

Substances

  • Antibodies, Fungal
  • Antigens, Fungal
  • Glycoproteins
  • Immunoglobulin A
  • Immunoglobulin G